Validation of a headspace solid-phase microextraction procedure with gas chromatography-electron capture detection of pesticide residues in fruits and vegetables

Mee Kin Chai, Guan Huat Tan

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Headspace solid-phase microextraction (HS-SPME) was evaluated for the determination of pesticide residues in fruits and vegetables by gas chromatography with an electron capture detector (GC-ECD). The fibre used was coated with polydimethylsiloxane (100 μm thickness) and the analytical conditions employed have been developed and optimised in a previous work [Chai, M. K., Tan, G. H., & Asha, L. (2008). Optimisation of headspace solid-phase microextraction for the determination of pesticide residues in vegetables and fruits. Analytical Sciences, 24 (2), 273-276]. The results show that the HS-SPME procedure gave a better linear range, accuracy, precision, detection and quantification limits and is adequate for analysing pesticide residues in fruits and vegetables. The average recoveries obtained for each pesticide ranged between 71% and 98% at three fortification levels with the relative standard deviation of less than 5%. Repeatability (0.3-3.7%) and intermediate precision (0.8-2.5%) were shown to be satisfactory. The limits of detection (0.01-1 μg L-1) and the limits of quantification (0.05-5 μg L-1) of these pesticides were much lower than the maximum residue levels (MRL), allowed for fruits and vegetables in Malaysia.

Original languageEnglish
Pages (from-to)561-567
Number of pages7
JournalFood Chemistry
Volume117
Issue number3
DOIs
Publication statusPublished - 01 Dec 2009

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Pesticide Residues
Solid Phase Microextraction
Vegetables
pesticide residues
Fruits
Gas chromatography
Gas Chromatography
Fruit
gas chromatography
vegetables
electrons
Electrons
fruits
Pesticides
Limit of Detection
pesticides
maximum residue limits
Malaysia
repeatability
detectors

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

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title = "Validation of a headspace solid-phase microextraction procedure with gas chromatography-electron capture detection of pesticide residues in fruits and vegetables",
abstract = "Headspace solid-phase microextraction (HS-SPME) was evaluated for the determination of pesticide residues in fruits and vegetables by gas chromatography with an electron capture detector (GC-ECD). The fibre used was coated with polydimethylsiloxane (100 μm thickness) and the analytical conditions employed have been developed and optimised in a previous work [Chai, M. K., Tan, G. H., & Asha, L. (2008). Optimisation of headspace solid-phase microextraction for the determination of pesticide residues in vegetables and fruits. Analytical Sciences, 24 (2), 273-276]. The results show that the HS-SPME procedure gave a better linear range, accuracy, precision, detection and quantification limits and is adequate for analysing pesticide residues in fruits and vegetables. The average recoveries obtained for each pesticide ranged between 71{\%} and 98{\%} at three fortification levels with the relative standard deviation of less than 5{\%}. Repeatability (0.3-3.7{\%}) and intermediate precision (0.8-2.5{\%}) were shown to be satisfactory. The limits of detection (0.01-1 μg L-1) and the limits of quantification (0.05-5 μg L-1) of these pesticides were much lower than the maximum residue levels (MRL), allowed for fruits and vegetables in Malaysia.",
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AB - Headspace solid-phase microextraction (HS-SPME) was evaluated for the determination of pesticide residues in fruits and vegetables by gas chromatography with an electron capture detector (GC-ECD). The fibre used was coated with polydimethylsiloxane (100 μm thickness) and the analytical conditions employed have been developed and optimised in a previous work [Chai, M. K., Tan, G. H., & Asha, L. (2008). Optimisation of headspace solid-phase microextraction for the determination of pesticide residues in vegetables and fruits. Analytical Sciences, 24 (2), 273-276]. The results show that the HS-SPME procedure gave a better linear range, accuracy, precision, detection and quantification limits and is adequate for analysing pesticide residues in fruits and vegetables. The average recoveries obtained for each pesticide ranged between 71% and 98% at three fortification levels with the relative standard deviation of less than 5%. Repeatability (0.3-3.7%) and intermediate precision (0.8-2.5%) were shown to be satisfactory. The limits of detection (0.01-1 μg L-1) and the limits of quantification (0.05-5 μg L-1) of these pesticides were much lower than the maximum residue levels (MRL), allowed for fruits and vegetables in Malaysia.

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