Simultaneous determination of Diazinon, Malathion and Quinalphos pesticide formulations by gas chromatography with an electron capture detector

Kumari Lal Asha, Guan Huat Tan, Mee Kin Chai

Research output: Contribution to journalArticle

Abstract

A gas chromatography (GC) method has been developed to analyze simultaneously separate formulations of Diazinon, Malathion and Quinalphos with the internal standard method. A mixture of pure standard solution spiked with Chlorpyrifos as the internal standard and diluted with methanol was sonicated to homogenize the solutions. A 2μL volume of the mixture standard solution was injected into the GC-ECD and a six point calibration curve that demonstrated a linear range was established for each target compound. Samples of each formulation, mixed with the internal standard and diluted with methanol were injected into the GC-ECD. Combinations of three different concentrations of each formulated pesticide were analyzed six times to obtain coefficients of variation which are less than 1%. The total analysis time for each run was 15 minutes. The concentration of active ingredients in the pesticide formulations were calculated from the peak area values at a retention time interpolated from the calibration graphs. The analytical results which show 55.41 ± 0.82% for Diazinon, 83.97 ± 0.36% for Malathion and 10.89 ± 0.89 % for Quinalphos were within the specifications for these commercial pesticide formulations.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalMalaysian Journal of Science
Volume25
Issue number2
Publication statusPublished - 01 Dec 2006

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Diazinon
Malathion
Pesticides
Gas chromatography
Detectors
Electrons
Methanol
Calibration
Chlorpyrifos
quinalphos
Specifications

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Simultaneous determination of Diazinon, Malathion and Quinalphos pesticide formulations by gas chromatography with an electron capture detector",
abstract = "A gas chromatography (GC) method has been developed to analyze simultaneously separate formulations of Diazinon, Malathion and Quinalphos with the internal standard method. A mixture of pure standard solution spiked with Chlorpyrifos as the internal standard and diluted with methanol was sonicated to homogenize the solutions. A 2μL volume of the mixture standard solution was injected into the GC-ECD and a six point calibration curve that demonstrated a linear range was established for each target compound. Samples of each formulation, mixed with the internal standard and diluted with methanol were injected into the GC-ECD. Combinations of three different concentrations of each formulated pesticide were analyzed six times to obtain coefficients of variation which are less than 1{\%}. The total analysis time for each run was 15 minutes. The concentration of active ingredients in the pesticide formulations were calculated from the peak area values at a retention time interpolated from the calibration graphs. The analytical results which show 55.41 ± 0.82{\%} for Diazinon, 83.97 ± 0.36{\%} for Malathion and 10.89 ± 0.89 {\%} for Quinalphos were within the specifications for these commercial pesticide formulations.",
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Simultaneous determination of Diazinon, Malathion and Quinalphos pesticide formulations by gas chromatography with an electron capture detector. / Asha, Kumari Lal; Tan, Guan Huat; Chai, Mee Kin.

In: Malaysian Journal of Science, Vol. 25, No. 2, 01.12.2006, p. 131-138.

Research output: Contribution to journalArticle

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