Preparation of antibodies to king cobra (Ophiophagus hannah) venom hemorrhagin and investigation of their cross-reactivity

Nget Hong Tan, Saifuddin M. Nomanbhay, M. I. Nik Jaafar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

N.-H. Tan, M. N. Saifuddin and M. I. Nik Jaafar. Preparation of antibodies to king cobra (Ophiophagus hannah) venom hemorrhagin and investigation of their cross-reactivity. Toxicon 28, 1355-1359, 1990.-Hannahtoxin, the major hemorrhagin purified from king cobra (Ophiophagus hannah) venom, elicits hemorrhages in rabbits but not in mice. Two antisera against hannahtoxin were prepared: one raised against purified hannahtoxin, while the other was raised against glutaraldehyde cross-linked and detoxified hannahtoxin. The antisera were refined by pepsin digestion and ammonium sulfate precipitation. They are of approximately equal potency in their ability to neutralize the hemorrhagic activity of king cobra venom in rabbits. The antisera did not form a precipitin line with venom of snakes of the Viperidae family nor neutralize hemorrhages elicited in mice by any of these venoms. However, when the hemorrhagic activity was assayed in rabbits, both antisera were able to abolish the hemorrhages elicited by all of the venoms tested. These results suggest that hannahtoxin displays few epitopes in common with hemorrhagins of viperid venoms, except those involved in the neutralization of hemorrhagic activity in rabbits. The epitopes of viperid venom hemorrhagins involved in the neutralization reaction in rabbits are different from those in mice.

Original languageEnglish
Pages (from-to)1355-1359
Number of pages5
JournalToxicon
Volume28
Issue number11
DOIs
Publication statusPublished - 01 Jan 1990

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Elapidae
Venoms
Antibodies
Immune Sera
Rabbits
Hemorrhage
Epitopes
Cobra Venoms
Precipitins
Snake Venoms
Pepsin A
Ammonium Sulfate
Glutaral
Viperidae
hemorrhagic proteinase IV
Digestion

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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title = "Preparation of antibodies to king cobra (Ophiophagus hannah) venom hemorrhagin and investigation of their cross-reactivity",
abstract = "N.-H. Tan, M. N. Saifuddin and M. I. Nik Jaafar. Preparation of antibodies to king cobra (Ophiophagus hannah) venom hemorrhagin and investigation of their cross-reactivity. Toxicon 28, 1355-1359, 1990.-Hannahtoxin, the major hemorrhagin purified from king cobra (Ophiophagus hannah) venom, elicits hemorrhages in rabbits but not in mice. Two antisera against hannahtoxin were prepared: one raised against purified hannahtoxin, while the other was raised against glutaraldehyde cross-linked and detoxified hannahtoxin. The antisera were refined by pepsin digestion and ammonium sulfate precipitation. They are of approximately equal potency in their ability to neutralize the hemorrhagic activity of king cobra venom in rabbits. The antisera did not form a precipitin line with venom of snakes of the Viperidae family nor neutralize hemorrhages elicited in mice by any of these venoms. However, when the hemorrhagic activity was assayed in rabbits, both antisera were able to abolish the hemorrhages elicited by all of the venoms tested. These results suggest that hannahtoxin displays few epitopes in common with hemorrhagins of viperid venoms, except those involved in the neutralization of hemorrhagic activity in rabbits. The epitopes of viperid venom hemorrhagins involved in the neutralization reaction in rabbits are different from those in mice.",
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Preparation of antibodies to king cobra (Ophiophagus hannah) venom hemorrhagin and investigation of their cross-reactivity. / Tan, Nget Hong; M. Nomanbhay, Saifuddin; Nik Jaafar, M. I.

In: Toxicon, Vol. 28, No. 11, 01.01.1990, p. 1355-1359.

Research output: Contribution to journalArticle

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