Optimization of fermentation parameters for bioethanol production from waste glycerol by microwave induced mutant Escherichia coli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biodiesel being one of the most promising alternative and renewable biofuels has lately seen rapid increase in its production capacity. Along with that there has also been a great increase in production of the co-product, crude glycerol. More attention is being paid to the utilization of crude glycerol from biodiesel production in order to defray the production cost of biodiesel and to promote biodiesel industrialization on a large scale. Bioconversion of crude glycerol to bioethanol through microbial fermentation is a very promising application of glycerol. The yield of bioethanol from fermentation of glycerol is greatly influenced by various parameters such as temperature, pH, glycerol concentration, organic nutrient concentration, and agitation speed and the bacterial species. The present study was undertaken to investigate optimum parameters for bioethanol production from raw glycerol (biodiesel by-product) by mutant Escherichia coli (E.coli) (ATCC11505) strain immobilized on chitosan optimized by Taguchi statistical method. The initial parameters were set each at four levels and the orthogonal array layout of L16 (45) was conducted. The result indicated that the significant controlling parameters for optimizing the operational fermentation were temperature 36°C, medium pH 7.0, initial glycerol concentration (250 g/l), and organic source concentration (5 g/l). The predicted value of bioethanol production under optimized conditions was (117.6 g/l). Immobilized cells are mainly used for economic benefits of continuous production or repeated use in continuous as well as in batch mode.

Original languageEnglish
Pages (from-to)1449-1461
Number of pages13
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Volume6
Issue number5
Publication statusPublished - 01 Jan 2015

Fingerprint

Bioethanol
Microwaves
Glycerol
Fermentation
Escherichia coli
Biofuels
Immobilized Cells
Bioconversion
Temperature
Chitosan
Nutrients
Byproducts
Statistical methods
Cells
Economics
Costs and Cost Analysis
Food

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

@article{474440deff0e46488a18d56941443117,
title = "Optimization of fermentation parameters for bioethanol production from waste glycerol by microwave induced mutant Escherichia coli",
abstract = "Biodiesel being one of the most promising alternative and renewable biofuels has lately seen rapid increase in its production capacity. Along with that there has also been a great increase in production of the co-product, crude glycerol. More attention is being paid to the utilization of crude glycerol from biodiesel production in order to defray the production cost of biodiesel and to promote biodiesel industrialization on a large scale. Bioconversion of crude glycerol to bioethanol through microbial fermentation is a very promising application of glycerol. The yield of bioethanol from fermentation of glycerol is greatly influenced by various parameters such as temperature, pH, glycerol concentration, organic nutrient concentration, and agitation speed and the bacterial species. The present study was undertaken to investigate optimum parameters for bioethanol production from raw glycerol (biodiesel by-product) by mutant Escherichia coli (E.coli) (ATCC11505) strain immobilized on chitosan optimized by Taguchi statistical method. The initial parameters were set each at four levels and the orthogonal array layout of L16 (45) was conducted. The result indicated that the significant controlling parameters for optimizing the operational fermentation were temperature 36°C, medium pH 7.0, initial glycerol concentration (250 g/l), and organic source concentration (5 g/l). The predicted value of bioethanol production under optimized conditions was (117.6 g/l). Immobilized cells are mainly used for economic benefits of continuous production or repeated use in continuous as well as in batch mode.",
author = "{M. Nomanbhay}, Saifuddin and H. Refal",
year = "2015",
month = "1",
day = "1",
language = "English",
volume = "6",
pages = "1449--1461",
journal = "Research Journal of Pharmaceutical, Biological and Chemical Sciences",
issn = "0975-8585",
publisher = "RJPBCS",
number = "5",

}

TY - JOUR

T1 - Optimization of fermentation parameters for bioethanol production from waste glycerol by microwave induced mutant Escherichia coli

AU - M. Nomanbhay, Saifuddin

AU - Refal, H.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Biodiesel being one of the most promising alternative and renewable biofuels has lately seen rapid increase in its production capacity. Along with that there has also been a great increase in production of the co-product, crude glycerol. More attention is being paid to the utilization of crude glycerol from biodiesel production in order to defray the production cost of biodiesel and to promote biodiesel industrialization on a large scale. Bioconversion of crude glycerol to bioethanol through microbial fermentation is a very promising application of glycerol. The yield of bioethanol from fermentation of glycerol is greatly influenced by various parameters such as temperature, pH, glycerol concentration, organic nutrient concentration, and agitation speed and the bacterial species. The present study was undertaken to investigate optimum parameters for bioethanol production from raw glycerol (biodiesel by-product) by mutant Escherichia coli (E.coli) (ATCC11505) strain immobilized on chitosan optimized by Taguchi statistical method. The initial parameters were set each at four levels and the orthogonal array layout of L16 (45) was conducted. The result indicated that the significant controlling parameters for optimizing the operational fermentation were temperature 36°C, medium pH 7.0, initial glycerol concentration (250 g/l), and organic source concentration (5 g/l). The predicted value of bioethanol production under optimized conditions was (117.6 g/l). Immobilized cells are mainly used for economic benefits of continuous production or repeated use in continuous as well as in batch mode.

AB - Biodiesel being one of the most promising alternative and renewable biofuels has lately seen rapid increase in its production capacity. Along with that there has also been a great increase in production of the co-product, crude glycerol. More attention is being paid to the utilization of crude glycerol from biodiesel production in order to defray the production cost of biodiesel and to promote biodiesel industrialization on a large scale. Bioconversion of crude glycerol to bioethanol through microbial fermentation is a very promising application of glycerol. The yield of bioethanol from fermentation of glycerol is greatly influenced by various parameters such as temperature, pH, glycerol concentration, organic nutrient concentration, and agitation speed and the bacterial species. The present study was undertaken to investigate optimum parameters for bioethanol production from raw glycerol (biodiesel by-product) by mutant Escherichia coli (E.coli) (ATCC11505) strain immobilized on chitosan optimized by Taguchi statistical method. The initial parameters were set each at four levels and the orthogonal array layout of L16 (45) was conducted. The result indicated that the significant controlling parameters for optimizing the operational fermentation were temperature 36°C, medium pH 7.0, initial glycerol concentration (250 g/l), and organic source concentration (5 g/l). The predicted value of bioethanol production under optimized conditions was (117.6 g/l). Immobilized cells are mainly used for economic benefits of continuous production or repeated use in continuous as well as in batch mode.

UR - http://www.scopus.com/inward/record.url?scp=84942032690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942032690&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 1449

EP - 1461

JO - Research Journal of Pharmaceutical, Biological and Chemical Sciences

JF - Research Journal of Pharmaceutical, Biological and Chemical Sciences

SN - 0975-8585

IS - 5

ER -