Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor

Y. Mustapha Kamil, M. H. Abu Bakar, M. A. Mustapa, M. H. Yaacob, N. H.Z. Abidin, A. Syahir, Hui Jing Lee, M. A. Mahdi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The surge of Dengue cases around the globe has intensified the demand for a reliable diagnostic method. The work demonstrates, to the best of our knowledge, the first label-free optical based sensor for detection of Dengue II E proteins. This was achieved by utilizing tapered optical fiber that has been functionalized with complementary recombinant antibodies. The fundamental concept of the sensor relies on the interaction between strong evanescent waves resulting from the dimensional change of the fiber and immune complex formed on the surface of the fiber when the virus is present. Sensitivity and detection limit values obtained with the sensor setup are 5.02 nm/nM and 1 pM, respectively, with a standard deviation value of no more than ±0.4. The compact and rapid sensor is a viable alternative for label-free and quantitative assessment of the infection, which may assist in providing better clinical management and understanding of the disease.

Original languageEnglish
Pages (from-to)820-828
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume257
DOIs
Publication statusPublished - 01 Mar 2018

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Fiber optic sensors
Labels
optical fibers
proteins
Proteins
sensors
Sensors
globes
fibers
Fibers
evanescent waves
viruses
infectious diseases
Antigen-Antibody Complex
antibodies
Viruses
Antibodies
Optical fibers
standard deviation
sensitivity

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Mustapha Kamil, Y., Abu Bakar, M. H., Mustapa, M. A., Yaacob, M. H., Abidin, N. H. Z., Syahir, A., ... Mahdi, M. A. (2018). Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor. Sensors and Actuators, B: Chemical, 257, 820-828. https://doi.org/10.1016/j.snb.2017.11.005
Mustapha Kamil, Y. ; Abu Bakar, M. H. ; Mustapa, M. A. ; Yaacob, M. H. ; Abidin, N. H.Z. ; Syahir, A. ; Lee, Hui Jing ; Mahdi, M. A. / Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor. In: Sensors and Actuators, B: Chemical. 2018 ; Vol. 257. pp. 820-828.
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Mustapha Kamil, Y, Abu Bakar, MH, Mustapa, MA, Yaacob, MH, Abidin, NHZ, Syahir, A, Lee, HJ & Mahdi, MA 2018, 'Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor', Sensors and Actuators, B: Chemical, vol. 257, pp. 820-828. https://doi.org/10.1016/j.snb.2017.11.005

Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor. / Mustapha Kamil, Y.; Abu Bakar, M. H.; Mustapa, M. A.; Yaacob, M. H.; Abidin, N. H.Z.; Syahir, A.; Lee, Hui Jing; Mahdi, M. A.

In: Sensors and Actuators, B: Chemical, Vol. 257, 01.03.2018, p. 820-828.

Research output: Contribution to journalArticle

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Mustapha Kamil Y, Abu Bakar MH, Mustapa MA, Yaacob MH, Abidin NHZ, Syahir A et al. Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor. Sensors and Actuators, B: Chemical. 2018 Mar 1;257:820-828. https://doi.org/10.1016/j.snb.2017.11.005