Isolation and characterization of a hemorrhagin from the venom of Ophiophagus hannah (king cobra)

Nget Hong Tan, Saifuddin M. Nomanbhay

Research output: Contribution to journalArticle

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Abstract

Nget Hong Tan and M. N. Saifuddin. Isolation and characterization of a hemorrhagin from the venom of Ophiophagus hannah (king cobra). Toxicon 28, 385-392, 1990.-The major hemorrhagin (termed hannahtoxin) of the venom of Ophiophagus hannah (king cobra) was purified to electrophoretic homogeneity by DEAE-Sephacel ion exchange chromatography, Sephadex G-200 gel filtration followed by a second DEAE-Sephacel chromatography. Proteolytic activity was associated with the hemorrhagic activity throughout the purification procedures. Hannahtoxin constituted approximately 2% of the crude venom. It had an isoelectric point of 5.3, a carbohydrate content of 12%, a mol. wt of 66,000 as determined by SDS-polyacrylamide gel electrophoresis and 63,000 as determined by gel filtration. It contains 1 mole of Zn per mole of protein. The minimum hemorrhage doses for hannahtoxin are 0.7 μg and 75 μg, respectively, in rabbits and in mice. Hannahtoxin was not lethal to mice at a dose of 2 mg/kg (i.v.) but killed rabbits at doses above 0.18 mg/kg (i.v.). It liberated protein from rabbit glomerular basement membrane but not rat glomerular basement membrane. Treatment of the hemorrhagin with EDTA and 1,10-phenanthroline eliminated both the proteolytic and hemorrhagic activities completely.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalToxicon
Volume28
Issue number4
DOIs
Publication statusPublished - 01 Jan 1990

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Elapidae
Venoms
Glomerular Basement Membrane
Chromatography
Rabbits
Gel Chromatography
Gels
Ion Exchange Chromatography
Isoelectric Point
Electrophoresis
Edetic Acid
Purification
Rats
Polyacrylamide Gel Electrophoresis
Ion exchange
Proteins
Carbohydrates
Hemorrhage
hemorrhagic proteinase IV
2-diethylaminoethanol

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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abstract = "Nget Hong Tan and M. N. Saifuddin. Isolation and characterization of a hemorrhagin from the venom of Ophiophagus hannah (king cobra). Toxicon 28, 385-392, 1990.-The major hemorrhagin (termed hannahtoxin) of the venom of Ophiophagus hannah (king cobra) was purified to electrophoretic homogeneity by DEAE-Sephacel ion exchange chromatography, Sephadex G-200 gel filtration followed by a second DEAE-Sephacel chromatography. Proteolytic activity was associated with the hemorrhagic activity throughout the purification procedures. Hannahtoxin constituted approximately 2{\%} of the crude venom. It had an isoelectric point of 5.3, a carbohydrate content of 12{\%}, a mol. wt of 66,000 as determined by SDS-polyacrylamide gel electrophoresis and 63,000 as determined by gel filtration. It contains 1 mole of Zn per mole of protein. The minimum hemorrhage doses for hannahtoxin are 0.7 μg and 75 μg, respectively, in rabbits and in mice. Hannahtoxin was not lethal to mice at a dose of 2 mg/kg (i.v.) but killed rabbits at doses above 0.18 mg/kg (i.v.). It liberated protein from rabbit glomerular basement membrane but not rat glomerular basement membrane. Treatment of the hemorrhagin with EDTA and 1,10-phenanthroline eliminated both the proteolytic and hemorrhagic activities completely.",
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Isolation and characterization of a hemorrhagin from the venom of Ophiophagus hannah (king cobra). / Tan, Nget Hong; M. Nomanbhay, Saifuddin.

In: Toxicon, Vol. 28, No. 4, 01.01.1990, p. 385-392.

Research output: Contribution to journalArticle

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