Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications

Nurulaina Fasya Saiful Anuar, Halina Misran, Musdalilah Ahmad Salim, Siti Zubaidah Othman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (νbend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 °C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection.

Original languageEnglish
Title of host publicationCurrent Trends in Materials Engineering
EditorsMohd Edeerozey Abd Manaf, T. Joseph Sahaya Anand, Md Nizam Abd Rahman, Radzali Othman, Mohd Sanusi Abdul Aziz
PublisherTrans Tech Publications Ltd
Pages67-71
Number of pages5
ISBN (Print)9783035710199
DOIs
Publication statusPublished - 01 Jan 2016
Event24th Scientific Conference of the Microscopy Society Malaysia, SCMSM 2015 - Malacca, Malaysia
Duration: 02 Dec 201504 Dec 2015

Publication series

NameKey Engineering Materials
Volume694
ISSN (Print)1013-9826

Other

Other24th Scientific Conference of the Microscopy Society Malaysia, SCMSM 2015
CountryMalaysia
CityMalacca
Period02/12/1504/12/15

Fingerprint

Fatty Alcohols
Enzyme immobilization
Glucose Oxidase
Glucose oxidase
Palm oil
Nanospheres
Silicon Dioxide
Silica
Glucose
palm oil
Glucose sensors
Silanes
Coulomb interactions
Benzene
Nanostructured materials

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Saiful Anuar, N. F., Misran, H., Ahmad Salim, M., & Othman, S. Z. (2016). Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications. In M. E. A. Manaf, T. J. S. Anand, M. N. A. Rahman, R. Othman, & M. S. A. Aziz (Eds.), Current Trends in Materials Engineering (pp. 67-71). (Key Engineering Materials; Vol. 694). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/KEM.694.67
Saiful Anuar, Nurulaina Fasya ; Misran, Halina ; Ahmad Salim, Musdalilah ; Othman, Siti Zubaidah. / Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications. Current Trends in Materials Engineering. editor / Mohd Edeerozey Abd Manaf ; T. Joseph Sahaya Anand ; Md Nizam Abd Rahman ; Radzali Othman ; Mohd Sanusi Abdul Aziz. Trans Tech Publications Ltd, 2016. pp. 67-71 (Key Engineering Materials).
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title = "Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications",
abstract = "In this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (νbend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 °C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection.",
author = "{Saiful Anuar}, {Nurulaina Fasya} and Halina Misran and {Ahmad Salim}, Musdalilah and Othman, {Siti Zubaidah}",
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Saiful Anuar, NF, Misran, H, Ahmad Salim, M & Othman, SZ 2016, Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications. in MEA Manaf, TJS Anand, MNA Rahman, R Othman & MSA Aziz (eds), Current Trends in Materials Engineering. Key Engineering Materials, vol. 694, Trans Tech Publications Ltd, pp. 67-71, 24th Scientific Conference of the Microscopy Society Malaysia, SCMSM 2015, Malacca, Malaysia, 02/12/15. https://doi.org/10.4028/www.scientific.net/KEM.694.67

Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications. / Saiful Anuar, Nurulaina Fasya; Misran, Halina; Ahmad Salim, Musdalilah; Othman, Siti Zubaidah.

Current Trends in Materials Engineering. ed. / Mohd Edeerozey Abd Manaf; T. Joseph Sahaya Anand; Md Nizam Abd Rahman; Radzali Othman; Mohd Sanusi Abdul Aziz. Trans Tech Publications Ltd, 2016. p. 67-71 (Key Engineering Materials; Vol. 694).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications

AU - Saiful Anuar, Nurulaina Fasya

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AU - Othman, Siti Zubaidah

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N2 - In this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (νbend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 °C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection.

AB - In this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (νbend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 °C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection.

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BT - Current Trends in Materials Engineering

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PB - Trans Tech Publications Ltd

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Saiful Anuar NF, Misran H, Ahmad Salim M, Othman SZ. Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications. In Manaf MEA, Anand TJS, Rahman MNA, Othman R, Aziz MSA, editors, Current Trends in Materials Engineering. Trans Tech Publications Ltd. 2016. p. 67-71. (Key Engineering Materials). https://doi.org/10.4028/www.scientific.net/KEM.694.67