Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli

Saifuddin M. Nomanbhay, Refal Hussain

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1 Citation (Scopus)

Abstract

Crude glycerol, an inevitable byproduct during biodiesel production, is emerging as a potential feedstock for fermentation, due to its availability and a reasonable price. The anaerobic digestion of glycerol derived from biodiesel manufacturing, in which COD was found to be 1010 g/kg, was studied in batch laboratory-scale reactors. Dissimilation of glycerol by Escherichia coli is strictly linked to their capacity to synthesize the highly reduced product 1,3-propanediol (1,3-PDO). The present study is focus on the development of adaptive mutant strains of Escherichia coli EC-MW (ATCC 11105), through microwave irradiation, at frequency 2.45 GHz and irradiation time 5 min pulse irradiation. The mutants were used for high bioethanol production from glycerol feedstock. Consequently, glycerol oxidative pathway (bioethanol) enhanced upon the parallel reduction in the 1,3-propanediol (1,3-PDO) pathway. The modified E. coli strains were able to increase bioethanol production upon fermentation reaching the level 280 g/L.

Original languageEnglish
Pages (from-to)210-222
Number of pages13
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Volume5
Issue number5
Publication statusPublished - 01 Jan 2014

Fingerprint

Bioethanol
Microwave irradiation
Microwaves
Glycerol
Escherichia coli
Biofuels
Fermentation
Feedstocks
Irradiation
Anaerobic digestion
Byproducts
Digestion
Availability

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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