# Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water

Ling Shing Wong, Simranjeet Kaur Judge, Betty Wan Niu Voon, Lee Jia Tee, Ka Yee Tan, Megalah Murti, Mee Kin Chai

Research output: Contribution to journalArticle

2 Citations (Scopus)

### Abstract

Anthropogenic activities cause serious metal pollution, and thus an effective screening method is important to indicate the presence of these pollutants in water. In this paper, a biosensor was developed to detect the presence of both heavy metals (Cu, Pb, and Cd) and light metals (Na, Al, and Li) in water. Microalgae chlorella vulgaris was used as the biological component, and the bioluminescence response of the microalgae to the metals was utilized as indication parameter (with $\lambda =350$-650 nm). The effects of pH, age of cell culture, and the density of the cells to the bioluminescence response were studied. From the experiment, the presence of these metals with the concentrations of 0.001-10.000 nm/L could be detected rapidly within 15 min of exposure. The usage of broad spectrum luminescence as indicator made the biosensor more cost effective. The sensitivity of the algae cells to the various environmental pollutants enabled the biosensor to respond to a wide range of metals within a short-period of exposure time. Furthermore, using non-genetically modified algae as biological component eluded strict regulations on the usage of genetic modified microbe in the field.

Original language English 8241379 2091-2096 6 IEEE Sensors Journal 18 5 https://doi.org/10.1109/JSEN.2017.2787786 Published - 01 Mar 2018

### Fingerprint

bioinstrumentation
Biosensors
Bioluminescence
bioluminescence
Algae
Metals
metals
water
Water
algae
contaminants
Light metals
Chlorella
Cell culture
Heavy metals
Luminescence
microorganisms
heavy metals
Screening
pollution

### All Science Journal Classification (ASJC) codes

• Instrumentation
• Electrical and Electronic Engineering

### Cite this

Wong, Ling Shing ; Judge, Simranjeet Kaur ; Voon, Betty Wan Niu ; Tee, Lee Jia ; Tan, Ka Yee ; Murti, Megalah ; Chai, Mee Kin. / Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water. In: IEEE Sensors Journal. 2018 ; Vol. 18, No. 5. pp. 2091-2096.
title = "Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water",
abstract = "Anthropogenic activities cause serious metal pollution, and thus an effective screening method is important to indicate the presence of these pollutants in water. In this paper, a biosensor was developed to detect the presence of both heavy metals (Cu, Pb, and Cd) and light metals (Na, Al, and Li) in water. Microalgae chlorella vulgaris was used as the biological component, and the bioluminescence response of the microalgae to the metals was utilized as indication parameter (with $\lambda =350$-650 nm). The effects of pH, age of cell culture, and the density of the cells to the bioluminescence response were studied. From the experiment, the presence of these metals with the concentrations of 0.001-10.000 nm/L could be detected rapidly within 15 min of exposure. The usage of broad spectrum luminescence as indicator made the biosensor more cost effective. The sensitivity of the algae cells to the various environmental pollutants enabled the biosensor to respond to a wide range of metals within a short-period of exposure time. Furthermore, using non-genetically modified algae as biological component eluded strict regulations on the usage of genetic modified microbe in the field.",
author = "Wong, {Ling Shing} and Judge, {Simranjeet Kaur} and Voon, {Betty Wan Niu} and Tee, {Lee Jia} and Tan, {Ka Yee} and Megalah Murti and Chai, {Mee Kin}",
year = "2018",
month = "3",
day = "1",
doi = "10.1109/JSEN.2017.2787786",
language = "English",
volume = "18",
pages = "2091--2096",
journal = "IEEE Sensors Journal",
issn = "1530-437X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "5",

}

Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water. / Wong, Ling Shing; Judge, Simranjeet Kaur; Voon, Betty Wan Niu; Tee, Lee Jia; Tan, Ka Yee; Murti, Megalah; Chai, Mee Kin.

In: IEEE Sensors Journal, Vol. 18, No. 5, 8241379, 01.03.2018, p. 2091-2096.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioluminescent Microalgae-Based Biosensor for Metal Detection in Water

AU - Wong, Ling Shing

AU - Judge, Simranjeet Kaur

AU - Voon, Betty Wan Niu

AU - Tee, Lee Jia

AU - Tan, Ka Yee

AU - Murti, Megalah

AU - Chai, Mee Kin

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Anthropogenic activities cause serious metal pollution, and thus an effective screening method is important to indicate the presence of these pollutants in water. In this paper, a biosensor was developed to detect the presence of both heavy metals (Cu, Pb, and Cd) and light metals (Na, Al, and Li) in water. Microalgae chlorella vulgaris was used as the biological component, and the bioluminescence response of the microalgae to the metals was utilized as indication parameter (with $\lambda =350$-650 nm). The effects of pH, age of cell culture, and the density of the cells to the bioluminescence response were studied. From the experiment, the presence of these metals with the concentrations of 0.001-10.000 nm/L could be detected rapidly within 15 min of exposure. The usage of broad spectrum luminescence as indicator made the biosensor more cost effective. The sensitivity of the algae cells to the various environmental pollutants enabled the biosensor to respond to a wide range of metals within a short-period of exposure time. Furthermore, using non-genetically modified algae as biological component eluded strict regulations on the usage of genetic modified microbe in the field.

AB - Anthropogenic activities cause serious metal pollution, and thus an effective screening method is important to indicate the presence of these pollutants in water. In this paper, a biosensor was developed to detect the presence of both heavy metals (Cu, Pb, and Cd) and light metals (Na, Al, and Li) in water. Microalgae chlorella vulgaris was used as the biological component, and the bioluminescence response of the microalgae to the metals was utilized as indication parameter (with $\lambda =350$-650 nm). The effects of pH, age of cell culture, and the density of the cells to the bioluminescence response were studied. From the experiment, the presence of these metals with the concentrations of 0.001-10.000 nm/L could be detected rapidly within 15 min of exposure. The usage of broad spectrum luminescence as indicator made the biosensor more cost effective. The sensitivity of the algae cells to the various environmental pollutants enabled the biosensor to respond to a wide range of metals within a short-period of exposure time. Furthermore, using non-genetically modified algae as biological component eluded strict regulations on the usage of genetic modified microbe in the field.

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